EmBioSys Lab
Publications

Publications

2026

M. Nandy, U. Ghosh, K. A. Ganar, H. Ippel, I. Dijkgraaf, and S. Deshpande.
pH-tunable material properties of glycine-rich condensates from tick bioadhesive
Advanced Functional Materials, 36:e15418, 2026.


2025

X. Shen, F. I. Hasyyati, K. Schroën, J. van der Oost§, and S. Deshpande§.
Microfluidic Approaches to Pickering Emulsions and Foams: Strategies, Challenges, and Promising Applications
Small, 21, e07603, 2025.

S. Shen, K. A. Ganar, J. van der Gucht§, and S. Deshpande§.
Cell-free screening of CRISPR-Cas activity by microfluidics-assisted in vitro compartmentalization
Trends in Biotechnology, 43(11):2926–2946, 2025.

C. Chen, C. M. Love, C. F. Carnahan, K. A. Ganar, A. N. Parikh, and S. Deshpande.
Regulating biocondensates within synthetic cells via segregative phase separation
ACS Nano, 19(22):20550–20563, 2025.

P. Vink, L. W. Honaker, and S. Deshpande.
Towards electrospray-assisted production of lipid-based synthetic cell assemblies
Soft Matter, 21:2977-2985, 2025.

K. A. Ganar, M. Nandy, P. Turbina, C. Chen, D. Suylen, E. Nihoul, E. L. Pascoe, S. van der Beelen, M. Plaum, L. van den Bos, C. J. M. Koenraadt, I. Dijkgraaf§, and S. Deshpande§.
Phase separation and ageing of glycine-rich protein from tick adhesive [Behind the paper] [News & Views]
Nature Chemistry, 17:186-197, 2025.

X. Shen, C. Chen, B. van der Meer, T. E. Kodger, U. Sen, S. Deshpande§, and J. van der Gucht§.
Understanding the Stability of Poorly Covered Pickering Emulsions Using on-Chip Microfluidics
Advanced Science, 2409903, 2025.

C. Zheng, Z. Hussain, C. Chen, R. J. de Haas, S. Deshpande, Z. Zhang, H. Zuilhof, and R. de Vries.
One-step antifouling coating of polystyrene using engineered polypeptides
Journal of Colloid and Interface Science, 685:350-360, 2025.


2024

K. Schroën, X. Shen, F. I. Hasyyati, S. Deshpande, and J. van der Gucht.
From theoretical aspects to practical food Pickering emulsions: Formation, stabilization, and complexities linked to the use of colloidal food particles
Advances in Colloid and Interface Science, 334:103321, 2024.

C. Chen, K. A. Ganar, R. J. de Haas, N. Jarnot, E. Hogeveen, R. de Vries, and S. Deshpande.
Elastin-like polypeptide coacervates as reversibly triggerable compartments for synthetic cells [Elveflow research summary]
Communications Chemistry, 7:198, 2024.

L. W. Honaker*, T-N. Gao*, K. R. de Graaf*, T. V. M. Bogaardt, P. Vink, T. Stürzer, G. Kociok-Köhn, H. Zuilhof§, F. M. Miloserdov§, and S. Deshpande§.
2D and 3D self-assembly of fluorine-free pillar-[5]-arenes and perfluorinated diacids at all-aqueous interfaces
Advanced Science, 2401807, 2024.

L. W. Honaker*, A. Eijffius*, L. Plankensteiner, C. V. Nikiforidis, and S. Deshpande.
Biosensing with oleosin-stabilized liquid crystal droplets
Small, 2309053, 2024.

N. Appelman, C. Chen, I. Gruppen, and S. Deshpande.
Interface-driven spontaneous differentiation-repulsion behaviour in isochemical droplet populations
Advanced Materials Interfaces, 11, 2300617, 2024.


2023

R. J. de Haas, K. A. Ganar, S. Deshpande, and R. de Vries.
pH-responsive elastin-like polypeptide designer condensates
ACS Applied Materials and Interfaces, 15(38):45336-45344, 2023.

K. Aryan, K. A. Ganar, S. Deshpande, R. M. Boom, and C. V. Nikiforidis.
Continuous counter-current electrophoretic separation of oleosomes and proteins from oilseeds
Food Hydrcolloids, 144:109053, 2023.

L. W. Honaker, J. Schaap, D. Kenbeek, E. Miltenburg, and S. Deshpande.
Heads or tails: investigating the effects of amphiphile features on the distortion of chiral nematic liquid crystal droplets
Journal of Materials Chemistry C, 11:4867–4875, 2023.

C. Chen, K. A. Ganar, and S. Deshpande.
On-chip octanol-assisted liposome assembly for bioengineering
Journal of Visualised Experiments, 193:e65032, 2023.


2022

C. Chen, H. Meng, T. Guo, S. Deshpande, and H. Chen.
Development of paper microfluidics with 3D-printed PDMS barriers for flow control
ACS Applied Materials and Interfaces, 14(35):40286–40296, 2022.

L. W. Honaker, C. Chen, F. M. H. Dautzenberg, S. Brugman, and S. Deshpande.
Designing biological microsensors with chiral nematic liquid crystal droplets
ACS Applied Materials and Interfaces, 14(33):37316–37329, 2022.

K. A. Ganar, L. Leijten, and S. Deshpande.
Actinosomes: Condensate-templated containers for engineering synthetic cells
ACS Synthetic Biology, 11(8):2869–2879, 2022.

E. Bouzetos, K. A. Ganar, E. Mastrobattista, S. Deshpande§, and J. van der Oost§.
(R)evolution-on-a-chip
Trends in Biotechnology, 40(1):60-76, 2022.


2021

K. A. Ganar, L. W. Honaker, and S. Deshpande.
Shaping synthetic cells through cytoskeleton-condensate-membrane interactions
Current Opinion in Colloid and Interface Science, 54:101459, 2021.

S. Deshpande§ and C. Dekker§.
Studying phase separation in confinement
Current Opinion in Colloid and Interface Science, 52:101419, 2021.


2020

M. G. F. Last*, S. Deshpande*, and C. Dekker.
pH-controlled coacervate-membrane interactions within liposomes.
ACS Nano, 14(4):4487-4498, 2020.


2019

S. Deshpande and C. Dekker.
Synthetic life on chip.
Emerging Topics in Life Sciences, ETLS20190097, 2019.

S. Deshpande, S. Wunnava, D. Hueting, and C. Dekker.
Membrane tension-mediated growth of liposomes.
Small, 15(38):e1902898, 2019.

F. Fanalista*, A. Birnie*, K. Charles, G. Pawlik, R. Maan, F. Burla, S. Deshpande, G. Koenderink, M. Dogterom, and C. Dekker.
Shape and size control of artificial cells for bottom-up biology.
ACS Nano, 13(5):5439–5450, 2019.

M. Schaich, J. Cama, K. Al Nahas, D. Sobota, K. Jahnke, S. Deshpande, C. Dekker, and U. F. Keyser.
An integrated microfluidic platform for quantifying drug permeation across biomimetic vesicle membranes.
Molecular Pharmaceutics, 16(6):2494–2501, 2019.

S. Deshpande, F. Brandenburg*, A. Lau*, M.G.F. Last, K. Spoelstra, S. Wunnava, L. Reese, M. Dogterom, and C. Dekker.
Spatiotemporal control of coacervate formation within liposomes.
Nature Communications, 10:1800, 2019.

K. Al Nahas, J. Cama, M. Schaich, K. Hammond, S. Deshpande, C. Dekker, M. G. Ryadnov, and U. F. Keyser.
A microfluidic platform for the characterisation of membrane active antimicrobials.
Lab on a Chip, 19(5):837–844, 2019.


2018

F. Fanalista*, S. Deshpande*, A. Lau, G. Pawlik, and C. Dekker.
FtsZ-induced shape transformation of coacervates.
Advanced Biosystems, 1800136, 2018.

S. Deshpande and C. Dekker.
On-chip microfluidic production of cell-sized liposomes.
Nature Protocols, 13:856–874, 2018.

S. Deshpande, W. K. Spoelstra, M. van Doorn, J. Kerssemakers, and C. Dekker.
Mechanical division of cell-sized liposomes.
ACS Nano, 12(3):2560–2568, 2018.

M. Kaiser*, F. Jug*, T. Julou*, S. Deshpande, T. Pfohl, O. Silander, G. Myers, and E. van Nimwegen.
Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software.
Nature Communications, 9:212, 2018.

W. K. Spoelstra*, S. Deshpande*, and C. Dekker
Tailoring the appearance: what will synthetic cells look like?
Current Opinion in Biotechnology, 51:47-56, 2018.


2017

I. Hug, S. Deshpande, K. Sprecher, T. Pfohl, and U. Jenal.
Second messenger-mediated tactile response by a bacterial rotary motor.
Science, 358:531–534, 2017.

S. Deshpande, A. Birnie, and C. Dekker.
On-chip density-based purification of liposomes.
Biomicrofluidics, 11:034106, 2017.


2016

M. Göllner, A. C. Toma, N. Strelnikova, S. Deshpande, and T. Pfohl.
A self-filling microfluidic device for noninvasive and time-resolved single red blood cell experiments.
Biomicrofluidics, 10:054121, 2016.

S. Deshpande, Y. Caspi, A. Meijering, and C. Dekker
Octanol-assisted liposome assembly on chip.
Nature Communications, 7:10447, 2016.


2015

Z. Swank, S. Deshpande, and T. Pfohl.
Trapping, entrainment and synchronization of semiflexible polymers in narrow, asymmetric confinements.
Soft Matter, 12:87–92, 2015.

A. Hochstetter, E. Stellamanns, S. Deshpande, S. Uppaluri, M. Engstler, and T. Pfohl.
Microfluidics-based single cell analysis reveals drug-dependent motility changes in trypanosomes.
Lab on a Chip, 15(8):1961–8, 2015.

S. Deshpande and T. Pfohl.
Real-time dynamics of emerging actin networks in cell-mimicking compartments.
PLoS ONE, 10(3):e0116521, 2015.


2012

S. Deshpande and T. Pfohl
Hierarchical self-assembly of actin in microconfinements using microfluidics.
Biomicrofluidics, 6:034120, 2012.


* denotes equal contribution, § denotes shared correspondence.